| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | East Hall HS | A | Carr | Buffington | Williams | Jones | 3:38.78 | East Jackson Senior Night |
| 4 | Monroe Area High School | A | Echols | Denmark | Jones | Battle | 3:32.57 | Monticello Invitational |
| 5 | Jefferson High School | A | Barnes | Schroeder | Collado | Perkins | 3:30.07 | Pachuta Insurance Track & Field |
| 6 | Oconee County High School | A | Qualmann | Beall | Barnes | Hale | 3:31.70 | Jefferson Invitational |
| 7 | Cherokee Bluff High School | A | Porter | Porter | Lund | Roberts | DNF | Hall County Track and Field Cham |