| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 478 | SANTOS-GONZALEZ, Jayden | SR | Oak Ridge |
3:10.00 | |
| 2 | 220 | SIDDIQI, Ali | SR | Winter Park |
3:57.18 | Winter Park - Wildcat Open 2023 |
| 3 | 104 | SOUZA, Alex | SO | West Orange |
3:58.32 | Winter Park - Wildcat Open 2024 |
| 4 | 286 | ALI, Shariz | SR | Winter Park |
NT | |
| 5 | 223 | BOLDEN, Ryan | JR | Winter Park |
NT | |
| 6 | 801 | BROWN, Nicholas | JR | Edgewater |
NT | |
| 7 | 570 | CABAN, Jeycob | SO | New Dimensions |
NT | |
| 8 | 321 | GARCIA, Omar | SR | Poinciana |
NT | |
| 9 | 305 | HITZEMAN, Harper | SR | Poinciana |
NT | |
| 10 | 477 | JEAN FRANCOIS, Jenel | JR | Oak Ridge |
NT | |
| 11 | 307 | LEE, Levi | SR | Poinciana |
NT | |
| 12 | 431 | LIGHTBOURN, Tyreeze | SO | Oak Ridge |
NT | |
| 13 | 457 | LONG, Javon | JR | Oak Ridge |
NT | |
| 14 | 81 | PREECE, Weston | JR | West Orange |
NT | |
| 15 | 203 | RAGLAND, Preston | FR | Winter Park |
NT | |
| 16 | 347 | REMLING, Shay | SO | Poinciana |
NT | |
| 17 | 128 | RODRIGUEZ, Joshua | SR | West Orange |
NT | |
| 18 | 549 | TOURKZI, Malik | SO | Deltona |
NT | |
| 19 | 152 | ZBYSZINSKI, Liem | JR | West Orange |
NT |