| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 958 | DAVIS, Jillian | FR | Apopka |
2:20.00 | |
| 2 | 732 | BICHANICH, Ella | SO | Edgewater |
2:35.00 | |
| 3 | 240 | SIDDIQI, Sofia | SO | Winter Park |
2:36.40 | Orange and Black |
| 4 | 737 | JAMES, Grace | SO | Edgewater |
2:41.19 | 2:41.19 Windermere National Invitational of Champions 2024 |
| 5 | 62 | MCCULLY, Emma | SO | Orangewood Christian |
2:41.84 | FHSAA 1A District 7 |
| 6 | 385 | BALZER, Ava | SO | Lake Brantley |
3:00.00 | |
| 7 | 731 | CHO, Boey | SO | Edgewater |
3:15.00 | |
| 8 | 807 | ROBERTS, Sebrina | FR | Edgewater |
3:30.00 | |
| 9 | 139 | BARIL, Keila | FR | West Orange |
NT | |
| 10 | 122 | GROULLI, Hanna | FR | West Orange |
NT | |
| 11 | 173 | HALL, Leta | SO | Legacy High School | NT | |
| 12 | 388 | MCCARTHY, Megan | FR | Lake Brantley |
NT | |
| 13 | 282 | MCCLEMENS, Kate | FR | Winter Park |
NT | |
| 14 | 672 | MUNOZ, Amaya | FR | University (Orlando) |
NT | |
| 15 | 213 | PAXMAN, Elaine | FR | Winter Park |
NT | |
| 16 | 709 | RAMIREZ, Lina | FR | University (Orlando) |
NT |