| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 4 | 681 | BARR-MILLER, Nyrania | SR | University (OC) |
NT | |
| 5 | 172 | MCCOY, Nyah | JR | West Orange |
NT | |
| 6 | 829 | YARED, Lindsey | JR | Trinity Prep |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 81 | APONTE, Elisa | FR | Orangewood Christian |
NT | |
| 3 | 258 | MYLES, Reilani | SO | University (Orlando) |
NT | |
| 4 | 180 | SMITH, Aniya | JR | West Orange |
NT | |
| 5 | 91 | EVERS, Mercedes | SO | Orangewood Christian |
NT | |
| 6 | 739 | BOTTOM, Amelia | SO | Bishop Moore |
NT | |
| 7 | 685 | ARROYO CANALES, Sarahi | JR | University (OC) |
NT | |
| 8 | 348 | FARWIG, Peyton | JR | Hagerty |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 366 | JAIME, Emiaj | JR | Hagerty |
NT | |
| 2 | 897 | WHITE, Bailey | JR | Geneva School |
NT | |
| 3 | 752 | DORCELY, Tessie | JR | Bishop Moore |
NT | |
| 4 | 898 | FLORES, Loralei | JR | Geneva School |
NT | |
| 5 | 875 | KNOX, Trinity | JR | Geneva School |
NT | |
| 6 | 798 | PATINO, Olivia | FR | Trinity Prep |
NT | |
| 7 | 358 | MORLEY, Alyssa | SR | Hagerty |
NT | |
| 8 | 265 | PENA, Alejandra | SO | University (Orlando) |
NT | |
| 9 | 776 | LEWIS, Jayonna | SO | Bishop Moore |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 839 | BURGOS, Addison | JR | Trinity Prep |
NT | |
| 2 | 5 | LOZADA DA SILVA, Anastasia | SO | First Academy (Orlando) |
NT | |
| 3 | 51 | AUGUSTI, Elizabeth | FR | First Academy (Orlando) |
NT | |
| 4 | 466 | BERNHARDT, Jessica | SR | Winter Park |
48.50 | |
| 5 | 500 | PHILLIPS, Shila | SO | Winter Park |
45.40 | |
| 6 | 455 | GOTAY, Julianna | JR | Winter Park |
45.50 | |
| 7 | 14 | DZIEKAN, Victoria | FR | First Academy (Orlando) |
51.00 | |
| 8 | 101 | DAVILA, Kristina | FR | Orangewood Christian |
NT | |
| 9 | 697 | BENJAMIN, Kayla | SR | University (OC) |
NT |