| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 656 | BRYANT, Jazlyn | FR | University (OC) |
NT | |
| 2 | 779 | REYES, Amara | SO | Bishop Moore |
NT | |
| 3 | 663 | ORR, Amya | SO | University (OC) |
NT | |
| 4 | 318 | COSTA, Casey | SR | Hagerty |
NT | |
| 5 | 943 | VOLTAIRE, Natalia | FR | Seminole (Sanford) |
1:24.00 | |
| 6 | 774 | POPAROD, Kennedy | SO | Bishop Moore |
1:33.13 | |
| 7 | 146 | ZENKEL, Haven | SO | West Orange |
NT | |
| 8 | 285 | JACOB, Neveah | SR | Hagerty |
NT | |
| 9 | 88 | MONAHAN, Olivia | FR | Orangewood Christian |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 635 | SUPERNAW, Julianna | JR | University (OC) |
1:23.82 | 5-Star Conference Championship |
| 2 | 89 | MCDONALD, Avery | SO | Orangewood Christian |
1:18.39 | FHSAA 1A District 7 |
| 3 | 46 | MASON, Calista | SR | First Academy (Orlando) |
1:13.95 | FHSAA 1A Region 2 |
| 4 | 993 | REID, Imani | SO | Seminole (Sanford) |
1:11.00 | |
| 5 | 48 | BROWN, Zahmya | SR | First Academy (Orlando) |
1:07.77 | FHSAA 1A Region 2 |
| 6 | 580 | DELA CRUZ, Victoria | SR | Boone |
1:10.00 | |
| 7 | 555 | ROWE, Livia | FR | Boone |
1:12.70 | |
| 8 | 353 | TRAPAGA MOREJON, Alyssa | JR | Hagerty |
1:15.05 | FHSAA 4A District 3 |
| 9 | 784 | BONNEWITZ, Paloma | JR | Bishop Moore |
1:18.55 |