| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GAZZOLI, jessica | Father Lopez | 2:55.00 |
| 2 | NUGENT, Anna | Father Lopez | 2:55.00 |
| 3 | REGISTER, Samantha | Master's Academy (Oviedo) | 2:59.00 |
| 4 | BOYLES, Paige | Melbourne | 3:00.47 |
| 5 | VARGAS, Brooke | East River | 3:02.25 |
| 6 | CASADO, liliana | West Orange | 3:02.75 |
| 7 | MCDOW, Cara | Geneva Classical Academy | 3:05.00 |
| 8 | KENNEDY, Holland | Geneva Classical Academy | 3:08.48 |
| 9 | NGO, Thu | East River | 3:08.73 |
| 10 | CACERES, Chase | Dr. Phillips | NT |
| 11 | DIERCKS, Johanna | Edgewater | NT |
| 12 | EATON, Riley | Horizon | NT |
| 13 | HENNEBERY, Jillian | Trinity Prep | NT |
| 14 | KLYCE, Alyssa | West Orange | NT |
| 15 | MADAI, Kyra | West Orange | NT |
| 16 | THOMPSON, Abigail | Trinity Prep | NT |
| 17 | WHITESELL, Maryn | Horizon | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | DENNEY, Olivia | Winter Park | 2:23.04 |
| 2 | CHRISTENSEN, Isabelle | Windermere | 2:25.00 |
| 3 | GROSS, Sarah | Winter Park | 2:26.41 |
| 4 | BERGSTROM, Caroline | Melbourne | 2:28.43 |
| 5 | ALEXANDER, Sabine | Boone | 2:30.00 |
| 6 | MINOR, Lauren | Windermere | 2:30.00 |
| 7 | FRANCA, Raissa | Horizon | 2:32.88 |
| 8 | BOURNE, Drayton | Boone | 2:34.95 |
| 9 | HERNDON, Ebony | Boone | 2:36.00 |
| 10 | MORTIMER, Cara | Trinity Prep | 2:36.32 |
| 11 | DURNEY, Kylie | Dr. Phillips | 2:40.00 |
| 12 | FEDER, Ella | Geneva Classical Academy | 2:42.00 |
| 13 | DUDEK, Charlotte | Windermere | 2:44.52 |
| 14 | WILSON, Ryleigh | Master's Academy (Oviedo) | 2:48.51 |
| 15 | MERLY, Jessica | Master's Academy (Oviedo) | 2:50.32 |
| 16 | YUNICK, Erin | Father Lopez | 2:51.21 |
| 17 | APOTSOS, Katelyn | Melbourne | 2:54.87 |