| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | NEJAME, Alessandra |  Lake Highland Prep | 
		NT | |
| 3 | SEIDU, Laila |  Horizon | 
		NT | |
| 4 | DA HORA, Lorena |  Freedom (Orlando) | 
		NT | |
| 5 | LOUISANT, Nijah |  West Orange | 
		NT | |
| 6 | TAYLOR, Lorrin |  Windermere Prep | 
		NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | GAUD, Liani |  Foundation Academy | 
		NT | |
| 2 | MONTAS, Hillary |  Lake Highland Prep | 
		NT | |
| 3 | AUGUSTE, Whitney |  Wekiva | 
		1:14.49 | Lake Mary Opening Classic 2024 | 
| 4 | BOURNE, Drayton |  Boone | 
		1:11.24 | Winter Park - Wildcat Open 2024 | 
| 5 | MITCHELL, Jazzlyn |  Olympia | 
		1:12.00 | |
| 6 | BROOKS, Zariya |  Freedom (Orlando) | 
		1:15.85 | Harmony Invitational | 
| 7 | EMILIEN, Marceline |  Olympia | 
		NT | |
| 8 | EADY, Zykeria |  Winter Park | 
		NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | PAREKH, Anisa |  Lake Highland Prep | 
		1:10.57 | Bishop Moore - Carl Friedland 2024 (Regional Qualifier) | 
| 2 | ROSENTHAL, Kate |  Foundation Academy | 
		1:09.95 | Windermere Meet of Champions Classic 2024 | 
| 3 | GEYER, Zoe |  Horizon | 
		1:07.96 | Windermere Meet of Champions Classic 2024 | 
| 4 | LONDON, Emelda |  Apopka | 
		1:04.60 | |
| 5 | CHAMBERS, Jayla |  Boone | 
		1:05.35 | Oak Ridge Invitational | 
| 6 | STONE, Abby |  Windermere Prep | 
		1:09.05 | |
| 7 | DAVIS, Aliyah |  Freedom (Orlando) | 
		1:10.46 | St. Cloud Invitational | 
| 8 | EARL, Kaley |  Foundation Academy | 
		1:11.15 | 2024 Be Like Ike Invitational (meet is full) | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | CASTEL, Destiny |  West Orange | 
		1:03.13 | Winter Park - Wildcat Open 2024 | 
| 2 | SARANITA, Chloe |  Windermere Prep | 
		1:02.92 | |
| 3 | GLOVER, Emani |  East Ridge | 
		1:01.95 | George Jenkins - Bob English Invitational 2024 | 
| 4 | SLAUGHTER, Amir |  East Ridge | 
		NT | |
| 5 | HICKS, Soriah |  Wekiva | 
		1:01.45 | Lake Mary Opening Classic 2024 | 
| 6 | SMITH, Amari |  Wekiva | 
		1:02.49 | Olympia Titans Invitational | 
| 7 | BENSON, Alexia |  East Ridge | 
		1:03.00 | |
| 8 | JONES, Kamari |  Apopka | 
		1:04.13 | Windermere Meet of Champions Classic 2024 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | ARRIOLA, Janecia |  Winter Park | 
		1:00.15 | Coach Miami Presents | 
| 2 | DENNEY, Olivia |  Winter Park | 
		59.73 | Star Athletics Sprint Series | 
| 3 | MILLER, Tabrea |  West Oaks | 
		58.45 | |
| 4 | HOWARD, Hannah-Rose |  West Oaks | 
		57.43 | |
| 5 | EDOUARD, Emigrace |  West Oaks | 
		58.00 | |
| 6 | MORILLO, Mariana |  Horizon | 
		59.00 | |
| 7 | ALEXANDER, Sabine |  Boone | 
		59.81 | Oak Ridge Invitational | 
| 8 | FISHER, Ayla |  West Orange | 
		1:00.92 | Winter Park - Wildcat Open 2024 |