| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | CARSHA, Campbell |  Windermere Prep | 
		NT | |
| 3 | CRAWFORD, Madison |  Horizon | 
		NT | |
| 4 | FERNANDEZ, Natalia |  Windermere Prep | 
		NT | |
| 5 | SCOTT, Xoie |  Freedom (Orlando) | 
		NT | |
| 6 | TROBVICH, Kate |  Lake Highland Prep | 
		NT | |
| 7 | MURPHY, McKenna |  Boone | 
		NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | PHILLIPS, Ma'Niah |  Wekiva | 
		NT | |
| 2 | DELA CRUZ, Victoria |  Boone | 
		NT | |
| 3 | WEEDEN, Laura |  Horizon | 
		NT | |
| 4 | CLARK, A'nylah |  West Oaks | 
		37.72 | Olympia Titans Invitational | 
| 5 | JIANG, Yingqian "Jasmine" |  Lake Highland Prep | 
		NT | |
| 6 | JOSEPH, Maryloucie |  Wekiva | 
		NT | |
| 7 | ALICEA, Lauren |  Lake Highland Prep | 
		NT | |
| 8 | PETERS, Layla |  Foundation Academy | 
		NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | HARDY, Ariel |  Boone | 
		31.64 | Oak Ridge Invitational | 
| 2 | SCOTT, Xaida |  Freedom (Orlando) | 
		31.35 | Harmony Invitational | 
| 3 | TEMPLETON, Avery |  Windermere Prep | 
		30.42 | |
| 4 | PIERRE, Jaslynn |  Winter Park | 
		30.18 | Winter Park - Wildcat Open 2024 | 
| 5 | CANDELARIO, Ariana |  East Ridge | 
		30.32 | Davenport - Bronco Invitational 2024 | 
| 6 | GORDON, Kayla |  Olympia | 
		30.51 | Windermere Meet of Champions Classic 2024 | 
| 7 | JEWELL, Faith |  East Ridge | 
		31.37 | Davenport - Bronco Invitational 2024 | 
| 8 | NARLOCH, Yasmyn |  Freedom (Orlando) | 
		35.96 | St. Cloud Invitational | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | EDOUARD, Emigrace |  West Oaks | 
		29.65 | Pre Season Time Trial 2024 | 
| 2 | LYN, Alahnie |  Apopka | 
		29.44 | Windermere Meet of Champions Classic 2024 | 
| 3 | GOTAY, Julianna |  Winter Park | 
		28.26 | 2023 Wildcat Open | 
| 4 | MEDLEY, Jada |  Olympia | 
		27.80 | |
| 5 | GRIGGS, Devuona |  Olympia | 
		28.00 | |
| 6 | SMITH, Aniya |  West Orange | 
		28.69 | Winter Park - Wildcat Open 2024 | 
| 7 | ANDERSON, Isabella |  East Ridge | 
		29.58 | George Jenkins - Bob English Invitational 2024 | 
| 8 | LAMBERT, Haley |  Foundation Academy | 
		29.87 | Olympia Titans Invitational | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | HICKS, Cebreeya |  Wekiva | 
		27.31 | Olympia Titans Invitational | 
| 2 | SIMMONS, Carmen |  Horizon | 
		27.01 | Windermere Meet of Champions Classic 2024 | 
| 3 | DENNEY, Olivia |  Winter Park | 
		26.20 | |
| 4 | MILLER, Tabrea |  West Oaks | 
		25.01 | |
| 5 | PETERS, Lee |  Foundation Academy | 
		26.02 | Olympia Titans Invitational | 
| 6 | FISHER, Ayla |  West Orange | 
		26.82 | Windermere Meet of Champions Classic 2024 | 
| 7 | CASTEL, Destiny |  West Orange | 
		27.22 | Winter Park - Wildcat Open 2024 | 
| 8 | LINARTES, Emma |  Apopka | 
		27.80 |