| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 3 |  Freedom (Orlando) | 
		A | 45.65 | Harmony Invitational | 
| 4 |  Boone | 
		A | NT | |
| 5 |  Windermere Prep | 
		A | DNS | 
| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 2 |  Horizon | 
		A | 44.95 | Windermere Meet of Champions Classic 2024 | 
| 3 |  Winter Park | 
		A | 43.50 | |
| 4 |  Apopka | 
		A | 42.73 | |
| 5 |  Olympia | 
		A | 42.81 | Windermere Meet of Champions Classic 2024 | 
| 6 |  West Orange | 
		A | 43.67 | Windermere Meet of Champions Classic 2024 | 
| 7 |  West Oaks | 
		A | 45.19 | Olympia Titans Invitational | 
| 8 |  Foundation Academy | 
		A | NT |