| Athlete | Team | Seed | Note | |
|---|---|---|---|---|
| 1 | DAVIS, Gary | West Orange |
57.43 | Windermere Meet of Champions Classic 2024 |
| 2 | KNIGHT, Jackson | Winter Park |
59.50 | |
| 3 | PARCHMENT, Keino | Apopka |
1:01.96 | Windermere Meet of Champions Classic 2024 |
| 4 | POSTEN, Jordan | Olympia |
1:02.00 | |
| 5 | BISSESSAR, Adam | West Orange |
1:02.20 | Winter Park - Wildcat Open 2024 |
| 6 | MCLEOD, Kyle | Boone |
1:03.00 | Oak Ridge Invitational |
| 7 | DEAN, Devin | Apopka |
1:03.00 | |
| 8 | CHRISTIAN, Jaheim | Olympia |
1:04.00 | |
| 9 | ROUNTREE, Zah'Monie | Olympia |
1:04.00 | |
| 10 | HARDY, Aaron | Boone |
1:04.78 | Oak Ridge Invitational |
| 11 | SIDDIQI, Ali | Winter Park |
1:04.94 | Winter Park - Wildcat Open 2024 |
| 12 | SANCHEZ, Alberto | Freedom (Orlando) |
1:06.36 | Harmony Invitational |
| 13 | PATTI, Aramis | Freedom (Orlando) |
1:06.93 | Harmony Invitational |
| 14 | BLACKWELL, Emmanuel | Horizon |
1:07.75 | Windermere Meet of Champions Classic 2024 |
| 15 | BANGERTER, Tristan | Freedom (Orlando) |
1:30.41 | Harmony Invitational |
| 16 | LIGHTNEAR, Gevont | East Ridge |
NT | |
| 17 | LAACK, James | East Ridge |
NT | |
| 18 | ALI, Sharize | Winter Park |
NT | |
| 19 | ESTERIL, Kimbilly | Wekiva |
NT |