| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cardinal Mooney |
A | Boeller | Borda | Jackson | Koch | DNF | Bobcat Relays |
| 2 | Village School |
A | Cavero | Dooney | Alvear | Slaboch | 58.46 | Village Quad |
| 3 | Babcock | A | Noel | Goshorn | Bonfig | Gomez | 56.83 | 10th Annual Loretta Purish Invitational |
| 4 | St. John Neumann |
A | Mejia | Le Quere | Martin-Gonzales | McFadden | 57.21 | CSN Invitational Powered by UnderArmour |
| 5 | Bonita Springs |
A | Findley | Javier-Soto | Martinez | Raphael | 58.88 | 60th Annual Eagle Invitational |
| 6 | Community School (Naples) |
A | Polit | Hagenbuckle | Ringhofer | Bersh | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lely |
A | Lozano-Palacios | Francois | Francois | Andris | 53.50 | CSN Invitational Powered by UnderArmour |
| 2 | Moore Haven |
A | Herrera | Ling | Gopher | Thomas | 51.50 | |
| 3 | Somerset Academy (PP) |
A | Carey | Reid | Jacob | King | 49.97 | Sam Burley Hall of Fame Invitational |
| 4 | First Baptist (Naples) |
A | Roth | Newman | Louzonis | Jones | 51.00 | |
| 5 | Aubrey Rogers | A | Pierre | Montayne | Joseph | Bershadski | 52.62 | Dunbar Tiger Classic (Approved Sanctioned Meet) |
| 6 | LaBelle |
A | White | Sandoval | Esquivel | Wheatle | 55.53 | Bishop Verot - Lady Viking Classic 2024 |