| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Windermere Prep |
A | 8:20.12 | |
| 2 | Trinity Prep |
A | 8:22.58 | FHSAA Class 2A Track & Field State Championships |
| 3 | First Academy (Orlando) |
A | 8:35.00 | |
| 4 | Lake Howell |
A | DNS | 8:48.08 Charlie Harris Relays |
| 5 | Father Lopez |
A | 9:16.98 | FHSAA 1A Region 2 |
| 6 | Victory Christian (LL) |
A | 9:23.35 | Lakeland City Championships @ George Jenkins 2025 |
| 7 | Geneva School |
A | 9:25.00 | |
| 8 | Cornerstone Charter |
A | 10:00.31 | ROYAL INVITATIONAL @ The First Academy 2025 |