| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | First Academy (Orlando) |
B | Brinkley | Wildenburg | Visser | Bissonnette | NT | |
| 4 | Lake Mary |
B | White | Little | Little | Walker | NT | |
| 5 | Bishop Moore |
B | Ferrufino | Estrada | Browne | Sullivan | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Father Lopez |
B | Tishler | Curtis | Depablos-Herrera | Carmichael | 52.00 | |
| 2 | Victory Christian (LL) |
B | McCarty | Arroyo | Earby | Johnson | 46.00 | |
| 3 | Cornerstone Charter |
A | Marsingill | Mendez | Vazques-Marquez | Turner | 47.59 | |
| 4 | Geneva School |
A | Alilin | Davis | Lauretta | Mitchell | 45.20 | Geneva Classic 2025 |
| 5 | Trinity Prep |
A | Saraiva | James | Davis-Rocha | Davidescu | 46.73 | Bishop Moore - Larry Kelly Invitational 2025 |
| 6 | Hagerty |
B | Pringle | Ashford | MacMillan | Davis | DNS | |
| 7 | Trinity Christian (Deltona) |
B | Leopold | Johnson | Weeks | Linton | 51.21 | |
| 8 | Geneva School |
B | Costar | Allen | Howell | Jho | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | First Academy (Orlando) |
A | Gardner | Hester | Ivsic | Tozin | 44.22 | FHSAA 1A Region 2 |
| 2 | Victory Christian (LL) |
A | Gray | Gray | Scott | McKinley | DNF | |
| 3 | Lake Mary |
A | Aristle | Crowder-Woods | Williams, Jr | Rosario | DNF | Lake Mary Invitational 2025 |
| 4 | Lake Howell |
A | Spells | Funnye' | Wilkerson | Quailey | 42.13 | Charlie Harris Relay |
| 5 | Bishop Moore |
A | Jusme | Talma | Gummer | Cantero | 42.26 | Bishop Moore - Larry Kelly Invitational 2025 |
| 6 | Hagerty |
A | Lopez | Hatfield | Gandy | Howell | 42.70 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 7 | Father Lopez |
A | Herdel | Hagen | Paredes | Svajko | 44.20 | FHSAA 1A Region 2 |
| 8 | Trinity Christian (Deltona) |
A | Baldwin | Brittain | Hudzik | Rodriguez | 44.83 | FHSAA 1A Region 2 |