| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Father Lopez |
B | NT | |
| 3 | Cornerstone Charter |
A | 5:45.00 | |
| 4 | Geneva School |
A | DNS | Lake Highland - Highlander Classic 2025 |
| 5 | Trinity Christian (Deltona) |
A | 5:10.51 | Deltona Invitational 2025 |
| 6 | Bishop Moore |
B | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Hagerty |
A | 4:30.00 | |
| 2 | Lake Howell |
A | 4:19.10 | FHSAA 4A District 3 |
| 3 | Father Lopez |
A | 4:18.02 | Bears Invitational (Regional Qualifier) |
| 4 | First Academy (Orlando) |
A | 4:09.30 | 4:09.30 Citrus League Championship 2024 |
| 5 | Lake Mary |
A | DQ | FHSAA 4A District 3 |
| 6 | Victory Christian (LL) |
A | 4:19.00 | |
| 7 | Bishop Moore |
A | 4:22.65 | Bishop Moore - Larry Kelly Invitational 2025 |
| 8 | Circle Christian |
A | DNS |