| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lake Mary |
A | NT | |
| 2 | Cornerstone Charter |
A | NT | |
| 3 | Trinity Prep |
B | NT | |
| 4 | Geneva School |
B | NT | |
| 5 | Victory Christian (LL) |
B | 1:55.00 | |
| 6 | Trinity Christian (Deltona) |
B | NT | |
| 7 | Hagerty |
B | 1:47.00 | |
| 8 | Lake Mary |
B | DNS |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Trinity Christian (Deltona) |
A | NT | |
| 2 | Father Lopez |
A | NT | |
| 3 | Victory Christian (LL) |
A | 1:45.00 | |
| 4 | Lake Howell |
A | 1:40.51 | Charlie Harris Relay |
| 5 | First Academy (Orlando) |
A | 1:42.53 | Lake Nona - Tammy Lafayette Relays 2025 |
| 6 | Trinity Prep |
A | NT | |
| 7 | Hagerty |
A | DNS | |
| 8 | Geneva School |
A | NT |