| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Geneva School |
B | Howell | Brehm | Costar | Faith | NT | |
| 4 | Cornerstone Charter |
A | Cumbal | Qurban | Just | Reis | 4:00.00 | |
| 5 | Bishop Moore |
B | Snyder | Browne | Dupas | Rowan | NT | |
| 6 | Lake Mary |
B | Wilson | Ballantyne | Justice | Kinnear | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | First Academy (Orlando) |
A | Jebailey | Wildenburg | McAndrew | Bissonnette | 3:48.50 | FHSAA 1A Region 2 |
| 2 | Lake Howell |
A | Oviedo | Eason | Williams | Patterson | 3:42.14 | Charlie Harris Relay |
| 3 | Hagerty |
A | Lapierre | Lapierre | Sauvao | Fitzgerald | DNS | |
| 4 | Lake Mary |
A | Robinson | Hartsfield | Dixon | Jones | 3:28.65 | FHSAA 4A District 3 |
| 5 | Victory Christian (LL) |
A | Williams | Bernard | Arroyo | Philemon | 3:36.83 | Shorecrest Senior Night |
| 6 | Bishop Moore |
A | Talma | Jenkins | Jusme | Cantero | 3:41.22 | Bishop Moore - Larry Kelly Invitational 2025 |
| 7 | Geneva School |
A | Liguori | Hendricks | Davis | Mitchell | 3:47.00 | |
| 8 | Father Lopez |
A | Watkins | Gorman | Hoe | Carmichael | 3:56.93 | 3:56.93 Trinity Prep - Saints Invitational 2024 |