| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lake Mary |
A | 7:54.11 | FHSAA Class 4A Track & Field State Championships |
| 2 | Hagerty |
A | 8:08.04 | 8:08.04 Lake Mary Invitational 2023 |
| 3 | Windermere Prep |
A | 8:13.48 | FHSAA Class 2A Track & Field State Championships |
| 4 | Trinity Prep |
A | 8:22.58 | FHSAA Class 2A Track & Field State Championships |
| 5 | Bishop Moore |
A | 8:28.51 | 8:28.51 Saints Invitational 2023 |
| 6 | Circle Christian |
A | 8:28.96 | FHSAA 1A District 7 |
| 7 | Winter Park |
A | 9:00.50 | |
| 8 | Victory Christian (LL) |
A | 9:15.76 | FHSAA 1A Region 3 |
| 9 | Windermere Prep |
B | DNS | |
| 10 | Cornerstone Charter |
A | 10:29.37 | East River Falcon Takeoff |
| 11 | Lake Mary |
B | NT |