| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Bishop Moore |
B | NT | |
| 4 | Hagerty |
B | DNS | |
| 5 | Lake Mary |
B | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Trinity Prep |
A | NT | |
| 2 | Cornerstone Charter |
A | 5:10.00 | |
| 3 | Lake Mary |
A | 4:20.00 | |
| 4 | Winter Park |
A | 4:10.50 | |
| 5 | Hagerty |
A | 4:18.00 | |
| 6 | Circle Christian |
A | 4:33.39 | 4:33.39 Saints Invitational 2023 |
| 7 | Windermere Prep |
A | DNS | |
| 8 | Bishop Moore |
A | NT |