| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 5 | Winter Park |
B | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lake Mary |
B | NT | |
| 2 | Bishop Moore |
B | NT | |
| 3 | Victory Christian (LL) |
B | 59.00 | |
| 4 | Windermere Prep |
A | 54.65 | |
| 5 | Hagerty |
B | 54.00 | |
| 6 | Windermere Prep |
B | 1:00.32 | |
| 7 | Trinity Prep |
B | NT | |
| 8 | Circle Christian |
B | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Winter Park |
A | 53.50 | |
| 2 | Circle Christian |
A | 52.07 | FHSAA 1A Region 2 |
| 3 | Lake Mary |
A | 50.40 | |
| 4 | Hagerty |
A | 49.26 | |
| 5 | Bishop Moore |
A | 50.34 | FHSAA Class 2A Track & Field State Championships |
| 6 | Victory Christian (LL) |
A | 51.00 | |
| 7 | Trinity Prep |
A | 52.64 | 52.64 Trinity Prep Relays 2023 |
| 8 | Cornerstone Charter |
A | 54.46 | Timber Creek Quad Meet |