| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Hagerty | B | NT | |
| 3 | Lake Highland Prep | B | NT | |
| 4 | Lake Mary | B | NT | |
| 5 | Trinity Prep | B | NT | |
| 6 | East Ridge | B | NT | |
| 7 | Foundation Academy | B | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | University (OC) | A | DNS | |
| 3 | Circle Christian | A | NT | |
| 4 | Seminole (Sanford) | B | DNS | |
| 5 | Geneva School | A | NT | |
| 6 | Foundation Academy | A | NT | |
| 7 | Windermere Prep | A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Lake Highland Prep | A | 1:51.71 | Spring Break Invitational at IMG |
| 3 | Trinity Prep | A | 1:49.00 | |
| 4 | Lake Mary | A | 1:20.00 | |
| 5 | Seminole (Sanford) | A | 1:43.84 | |
| 6 | Hagerty | A | 1:51.00 | |
| 7 | East Ridge | A | 1:51.96 | Florida Relays |