| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WOOD, Ayah | Dalton School | NT | |
| 3 | RODRIGUEZ, Jaylah | Winter Park |
1:37.86 | Bishop Moore - Carl Friedland Invite 2025 |
| 4 | CHRISTIE-WARD, Joie | Dalton School | 1:10.00 | |
| 5 | SAINTFLEUR, Taraji | Ocoee |
1:15.06 | Timber Creek Elite Classic 2025 |
| 6 | REUSS, Kensington | Winter Park |
1:46.25 | Timber Creek Elite Classic 2025 |