| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Lake Region | B | 58.20 | |
| 3 | Auburndale | B | NT | |
| 4 | Lake Wales | B | NT | |
| 6 | George Jenkins | B | DNS | |
| 7 | George Jenkins | C | DQ |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Haines City | A | 54.32 | Poinciana Relays |
| 3 | Auburndale | A | 51.60 | |
| 4 | Lake Wales | A | 48.32 | Berkley Track and Field Invitational |
| 5 | George Jenkins | A | 48.94 | FSU Relays (High Schools) |
| 6 | Florida Prep | A | 54.07 | St. Cloud Invitational |
| 7 | Lake Region | A | NT |