| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Lake Region | B | NT | |
| 4 | Lake Wales | B | NT | |
| 5 | Lake Region | A | NT | |
| 6 | George Jenkins | B | 1:55.00 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Haines City | A | 1:49.08 | Walt Disney World Invitational |
| 4 | Auburndale | A | 1:45.00 | |
| 5 | George Jenkins | A | 1:48.00 | |
| 6 | Lake Wales | A | 1:52.00 | |
| 7 | Florida Prep | A | NT |