| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 130 | POOL, Gimel | SO | Ridge Community |
NT | |
| 3 | 668 | TURNER, Payton | FR | Frostproof |
NT | |
| 4 | 308 | HAYNES, Temia | SO | Lake Region |
1:34.27 | Lake Region Sprint Hurdle Night 2024 |
| 5 | 410 | TAYLOR, Keyani | JR | Hardee |
NT | |
| 6 | 120 | ARNOLD, Natalie | FR | Ridge Community |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 339 | PIERRE, Joanna | SR | Lake Region |
1:31.25 | George Jenkins - Bob English Invitational 2024 |
| 2 | 181 | LEWIS, Kaitlyn | SO | Auburndale |
1:18.49 | Lake Region Sprint Hurdle Night 2024 |
| 3 | 89 | ROMEUS, Stefanie | SR | Haines City |
1:16.14 | Berkeley Track and Field Invitational |
| 4 | 666 | VASQUEZ, Yalimar | JR | Frostproof |
1:08.00 | |
| 5 | 328 | PIERRE GILLES, Sanaya | JR | Lake Region |
1:12.72 | Lake Region Sprint Hurdle Night 2024 |
| 6 | 213 | PAYOUTE, Wiseberly | FR | Auburndale |
1:16.66 | Winter Haven Classic |
| 7 | 368 | WINFREY, Jadyn | FR | Hardee |
1:30.46 | Larry Richardson Memorial Invite |
| 8 | 301 | MARTINEZ, Brianna | JR | Lake Region |
1:33.81 | Winter Haven Classic |