| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | FUCA, Gina | 2:25.76 | |
| 2 | WOLTJER, Emma | Southeastern U. |
2:26.00 |
| 3 | ROHM, Natalie | Southeastern U. |
2:29.00 |
| 4 | FEDER, Grace | Southeastern U. |
2:29.19 |
| 5 | VORKAPICH, Kaitlyn | Southeastern U. |
2:29.59 |
| 6 | DAVIS, Mariam | UNAT-Southeastern U. | 2:30.00 |
| 7 | BAKER, Abbie | Southeastern U. |
2:30.50 |
| 8 | O'REGAN, Riley | Tampa |
2:32.00 |
| 9 | CARPENTER, Grace | Southeastern U. |
2:34.00 |
| 10 | CAMP, Eva | Southeastern U. |
2:35.00 |
| 11 | MACEDO, Kathy | Southeastern U. |
2:37.16 |
| 12 | KULIS, Faith | Palm Beach Atlantic |
2:45.04 |
| 13 | SLAGEL, Sydney | Southeastern U. |
3:06.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BROWN, Carolyn | 2:12.00 | |
| 2 | LA-ROSE, Arden | 2:12.00 | |
| 3 | VALDEZ, Victoria | 2:16.50 | |
| 4 | MAHONY, Aoife | Palm Beach Atlantic |
2:17.00 |
| 5 | WYATT, Alyssa | 2:18.92 | |
| 6 | JOHNSON, Grace | 2:20.00 | |
| 7 | KOLODGE, Cora | 2:20.00 | |
| 8 | SILVA, Ashley | 2:22.33 | |
| 9 | BLASER, Jillian | Southeastern U. |
2:23.00 |
| 10 | KRINEY, Emily | Tampa |
2:23.39 |
| 11 | DEITRICK, Katelyn | Tampa |
2:23.50 |
| 13 | FRANCE, Jasmine | My Brother's & Sister's Keeper | 2:24.73 |