| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MALMGREN, Parker | Tampa |
50.50 |
| 2 | CLARKE, Phillip | Southeastern U. |
50.09 |
| 3 | KICHUK, Eamonn | Toronto Blue Devils | 48.80 |
| 4 | HAYDEN, Alexander | O.T.S. Speed | 48.37 |
| 5 | BLUNT, Malik | Southeastern U. |
48.80 |
| 6 | COULTER, Joey | Tampa |
48.85 |
| 7 | JORDAN, Calvin | Tampa |
50.21 |
| 8 | CRAWFORD, Zakaiel | Southeastern U. |
51.09 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SALOMON, Don | Southeastern U. |
53.13 |
| 2 | RUSSO, AJ | Tampa |
52.88 |
| 3 | REEVES, Luke | Florida Southern |
52.22 |
| 4 | EHRLICH, Ethan | Tampa Track Club |
51.50 |
| 5 | PELLETIER, Riley | Tampa |
52.02 |
| 6 | YISRAEL, Meshahraimanuel | MYis Athletics | 52.50 |
| 7 | COLON, Antonio | Tampa |
53.03 |
| 8 | PELKEY, Tyler | Tampa |
53.14 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GLOY, Julian | Unattached | 55.07 |
| 2 | BEDFORD, Nikko | Tampa |
54.78 |
| 3 | FRIEND, Kyle | Tampa |
54.06 |
| 4 | FAUGHT, Max | Tampa |
53.29 |
| 5 | KHASHOU, Jacob | Tampa |
53.60 |
| 6 | LEBRON, Sebastian | Southeastern U. |
54.30 |
| 7 | DEHUT, Zach | Florida College | 55.00 |
| 8 | LOPEZ, Eric | Unattached | 1:02.00 |