| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Tohopekaliga |
A | Sauri Colon | Maragh | Bicy | Richey | 11:45.62 | Harmony Invitational |
| 2 | First Academy (Orlando) |
A | Alvarez | Cruz Arroyo | Stewart | Guss | NT | |
| 3 | Lake Nona |
A | Mendez | Ramos Rodrigues | Chao | Malaykhan | NT | |
| 4 | Lake Nona |
B | Becherer | Moreira de Souza | Tabares | Nelson | NT | |
| 5 | Windermere |
A | Scott | QUAN | Barber | Crevasse | NT | |
| 6 | Windermere |
B | Dudek | Goote | Hixson | Christensen | NT |