| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | First Academy (Orlando) |
A | Jebailey | Faustino | McAndrew | Fouraker | 8:35.00 | |
| 2 | Windermere |
A | Chakrouni | Rossi | Valery Mendez | Tasior | 8:36.70 | 8:36.70 Lake Buena Vista Home Meet |
| 3 | Colonial |
A | Thompson | Sanders | Alvarez | Jordan | 8:51.91 | FHSAA 4A Region 1 |
| 4 | Tohopekaliga |
A | Thou | Petithomme | Pena | Gutierrez | 9:01.36 | Harmony Invitational |
| 5 | Lake Nona |
B | Ayure | Lambert | Thomas | Fagan | 9:10.00 | |
| 6 | Innovation_Orlando | A | Musouic | Iskander | Gooden | Davila | 9:32.66 | Harmony Invitational |
| 7 | Windermere |
B | Ford | Lee | Musarskiy | Reed | DNS | |
| 8 | Innovation_Orlando | B | Bianchi | Pascua | Quinlan | Gill | DNS | |
| 9 | Lake Nona |
A | Pantoja | Bogan | Gonzalez | Gonzalez | NT |