| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lake Nona |
B | Jonassaint | Stokes | Martin | Henderson | NT | |
| 4 | Windermere |
B | Davis | Winslow | Cabezudo | Speed | DNS | |
| 5 | Innovation_Orlando | B | Hubbard | Caballero | Altidor Jr | Harris | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lake Nona |
A | Dove | Kimmons | Allen | Stokes | 1:37.04 | Poinciana Relays |
| 3 | Windermere |
A | George | Todd | Rogers | Bronaugh | 1:34.00 | |
| 4 | Colonial |
A | Mintz | Castaneda | Gonzalez | Scott | 1:26.00 | |
| 5 | First Academy (Orlando) |
A | Ivsic | Obatimilehin | Gardner | Powell | 1:32.00 | |
| 6 | Tohopekaliga |
A | Hall | Jones | Gbaa | Veras | NT | |
| 7 | Innovation_Orlando | A | Harris | Reyes | Iskander | Moore | DNS |