| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Holy Trinity |
A | Francis | Collins | Walter | Sarratt | DNS | |
| 4 | Cornerstone Charter |
A | Bossier | Crabb | Hierbe | Mitchell | DQ | |
| 5 | Foundation Academy |
A | Gaud | Earl | Watts | Rosenthal | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Victory Christian (LL) |
A | Choquette | Williams | Roetzel-Dube | Hunt | 4:10.64 | Charger Invitational |
| 2 | Pineapple Cove |
A | Goddard | Revis | Rodriguez | Norton | DNS | |
| 3 | Wekiva |
A | Scott | Smith | Hicks | Hicks | 4:04.75 | 2024 Hawks on the Hill Open |
| 4 | Montverde Academy |
A | Reed | Hodge | Hudson | Williams | DNS | |
| 5 | IMG Academy |
A | Kitt | Lowe | Nebel | Choice | DNS | |
| 6 | First Academy (Orlando) |
A | Brown | Claytor | Landri | Mason | NT | |
| 7 | Edgewater |
A | Hutchinson | Price | Wright | Sos | DQ | FHSAA 3A District 6 |