| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 2 |  Palm Beach Atlantic | 
		A | Boshka | Costes | Shamel | Nordmeyer | 4:28.58 | South Florida Collegiate Challenge Championships | 
| 3 |  Lynn | 
		A | Ramirez | Mullins | Koch | Dreibelbis | 4:20.00 | |
| 4 |  Tampa | 
		A | Sullivan | Hicks | Kruchoski | Wilson | 4:02.23 | Black and Gold Invite | 
| 5 |  Saint Leo | 
		A | Aguilar | Mahony | Boger | Dervan | 4:05.00 | |
| 6 |  Florida Southern | 
		A | Procopio | Mussante | Kortright | Goll | 4:22.00 |