| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LENO, Keylon | Southeastern U. |
52.30 | |
| 2 | DUNHAM, Carsen | Tampa Track Club |
52.10 | |
| 3 | PELLETIER, Riley | Tampa |
52.02 | Tampa Open 2025 |
| 4 | TOOHEY, Cade | Tampa |
51.89 | |
| 5 | DEWEERDT, Sam | Tampa |
51.90 | |
| 6 | STEWART, Cory | Unattached | 52.05 | |
| 7 | KHASHOU, Jacob | Tampa |
52.23 | |
| 8 | FAUGHT, Max | Tampa |
53.29 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MCGRORTY, Ian | Flagler |
51.83 | The Legends of Alachua County Invitational College TEAMS |
| 2 | MARTIN, Wade | Tampa |
51.40 | The Spartan Classic |
| 3 | CLARKE, Phillip | Southeastern U. |
49.70 | |
| 4 | EDWARDS, Malik | Flagler |
49.50 | |
| 5 | RALEIGH, Mccray | Warner |
49.63 | |
| 6 | SALOMON, Don | Southeastern U. |
50.95 | |
| 7 | KANU, Harry | Tampa Track Club |
51.77 | |
| 8 | RUIS, Luca | Tampa |
51.89 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CRAWFORD, Zakaiel | Southeastern U. |
49.25 | |
| 2 | WILLIAMSON III, James | Southeastern U. |
48.80 | |
| 3 | HARPER, Houston | Flagler |
48.50 | |
| 4 | TAYLOR, Joseph | UNAT-Southeastern U. | 47.95 | 2025 Sun Conference Indoor Championship |
| 5 | VASQUEZ, Omar | Warner |
47.95 | Sun Conference Outdoor Track & Field Championship |
| 6 | SOUTH, Malachi | Flagler |
48.50 | |
| 7 | CAMARA, Logan | Flagler |
49.00 | |
| 8 | CUNNINGHAM, Xander | Flagler |
49.50 |