| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PETELL, Zoe | Flagler |
29.00 | |
| 3 | MEZZIO, Sofia | Tampa Track Club |
28.20 | |
| 4 | VREELAND, Megan | Flagler |
27.64 | Embry-Riddle Eagle Invite |
| 5 | KELLY, Maya | Unattached | 28.18 | |
| 6 | MILLER, Sara | Flagler |
29.00 | |
| 7 | MCGOUGH, Courtney | Flagler |
29.39 | Celebration Pointe Classic College TEAMS |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ZINNIE, Zoe | Flagler |
27.00 | |
| 2 | KOWNICK, Lindsey | Tampa |
26.93 | Tampa Classic |
| 3 | CLAYTON, Marielle | Tampa |
26.72 | The Legends of Alachua County Invitational College TEAMS |
| 4 | MORRIS, Molly | Tampa |
26.43 | Tampa Open 2025 |
| 5 | LUNSFORD, Tatum | Tampa |
26.69 | Tampa Classic |
| 6 | YORK, Elizabeth | Tampa |
26.78 | Tampa Classic |
| 7 | MOSESSO, Ashley | Tampa |
26.99 | Tampa Open 2025 |
| 8 | THOMAS, Anna | Tampa |
27.35 | Celebration Pointe Classic College TEAMS |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GREEN, Chloe | Tampa |
26.23 | Tampa Classic |
| 2 | HICKS, Lillian | Tampa |
26.03 | Tampa Open 2025 |
| 3 | MENDEZ, Irmiris | Southeastern U. |
24.69 | 2025 Sun Conference Indoor Championship |
| 4 | CLARKE-BROOMFIELD, Jahhi | Warner |
24.21 | |
| 5 | DODSON, Latasha | Team Dodson Elite | 24.65 | |
| 6 | BONDS, Areeyanna | Southeastern U. |
25.30 | |
| 7 | CARTER, Kate | Tampa |
26.21 | |
| 8 | MURPHY, Allie | Tampa |
26.23 | Tampa Open 2025 |