| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | FIELDS, Alexis | Fivay |
NT | |
| 3 | REYES, Sheila | Fivay |
NT | |
| 4 | BROWN, Fredre'Niyah | Fivay |
24.72 | Coyote Invite Cypress Creek Wesley Chapel |
| 5 | BROWN, Fredre'lina | Fivay |
26.02 | Freedom Spring Break Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BONNEY, Tajinae | Gulf |
20.61 | Anclote Sharks Early Bait 2024 |
| 3 | VOVERIS, Olivia | Gulf |
19.31 | Mitchell Quad Meet #1 |
| 4 | DENNING, Nandi | Wesley Chapel |
17.50 | FHSAA 3A Region 2 |
| 5 | GARCIA, Charlize | Wesley Chapel |
18.70 | Anclote Sharks Early Bait 2024 |
| 6 | JANKOVIC, Maya | Bishop McLaughlin |
20.51 | Raven Invitational 2024 (FHSAA Sanctioned)) |
| 7 | DIXON, Gabrielle | Anclote |
21.38 | Berkeley Track and Field Invitational |