| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | JILES, Sofia | Lake Mary | 26.87 |
| 2 | IVA, Kylie | Lake Mary | 26.50 |
| 3 | BARR-MILLER, Nyrania | University (OC) | 25.98 |
| 4 | ALLEN, Sidney | Seminole (Sanford) | 25.24 |
| 5 | SOREMI, Oludoyin | Seminole (Sanford) | 25.32 |
| 6 | SOREMI, Oluwadara | Seminole (Sanford) | 26.27 |
| 7 | LOWE, Mikiya | Lake Howell | 26.77 |
| 8 | STOKES, Ava | Lake Howell | 27.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | TODMAN, Lavona | University (OC) | 32.42 |
| 2 | LANE, Kaylyn | Lake Brantley | 29.11 |
| 3 | FARWIG, Peyton | Hagerty | 28.76 |
| 4 | JAIME, Emiaj | Hagerty | 27.72 |
| 5 | BROOKS, Monica | Lake Mary | 27.90 |
| 6 | PAYAMPS, Elise | Hagerty | 28.93 |
| 7 | WHITE, Bailey | Geneva School | 31.61 |
| 8 | CORTES, Aliyah | Lyman | 32.86 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | HOWARD, Madalyn | Oviedo | NT |
| 2 | HUAMAN, Scarlett | Winter Springs | NT |
| 3 | BENJAMIN, Kayla | University (OC) | NT |
| 4 | BENDER, Alexa | Lake Brantley | NT |
| 5 | WILLIAMS, AANASTASIA | Crooms | NT |
| 6 | ALLO, Sydney | Winter Springs | NT |
| 7 | NORFLEET, Nalani | Lyman | NT |
| 8 | SCOTT-BERNARD, Saudia | Lake Howell | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | PEREZ, Alondra | Crooms | NT |
| 2 | FLORES, Loralei | Geneva School | NT |
| 3 | CUNNINGHAM, Zen | Winter Springs | NT |
| 4 | LAWSON, Jasmine | Oviedo | NT |
| 5 | HAWK, Cheyanne | Oviedo | NT |
| 6 | BARKER, Melini | Crooms | NT |
| 7 | BROWN, Jordan | Lake Brantley | NT |