| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Lake Brantley | A | Harris | Woodson | Williams | Davis | 44.07 |
| 3 | Oviedo | A | Hernandez | Washington | Williams | Johnson | 43.29 |
| 4 | Lake Mary | A | Mitchell | Woodward | Gant | Marshall | DNS |
| 5 | Lake Howell | A | Quaily | Forbes | Stell | Morris | DNS |
| 6 | Seminole (Sanford) | A | Berchal | Campbell | Croom II | Griffin | 43.77 |
| 7 | Hagerty | A | Henson | Ngyuen | Shyy | Piris | 45.02 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Geneva School | A | Alilin | Credle | White | Hendrix | 46.25 |
| 4 | Lyman | A | Beason | Bouey | Grimes | Grimes | 45.48 |
| 5 | University (OC) | A | Irwin | Henry | Hayes | Stuart | DNS |