| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GIAMBALVO, Ella | Winter Springs | 2:24.04 |
| 2 | JACOBUS, Olivia | Oviedo | 2:28.37 |
| 3 | BRANHAM, Emily | Lake Mary | 2:34.09 |
| 4 | FALK, Madalyn | Oviedo | 2:35.00 |
| 5 | LITZ, Sadie | Winter Springs | 2:38.60 |
| 6 | RAESLY, Ella | Geneva School | 2:39.75 |
| 7 | MCCLELLAND, Caroline | Lake Mary | 2:40.53 |
| 8 | DAVID, Kailyn | Hagerty | 2:41.90 |
| 9 | ATWELL, Hannah | Oviedo | 2:42.02 |
| 10 | LOZADA CARRILLO, Mariaangel | Seminole (Sanford) | 2:42.47 |
| 11 | MCKAY, Ava | Lake Mary | 2:44.62 |
| 12 | MEYER, Veronica | University (OC) | 2:45.00 |
| 13 | SURESH KUMAR, Maunickaa | Seminole (Sanford) | 2:45.69 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | TYLER, Amaya | Lake Howell | 2:47.12 |
| 2 | VARGAS, Jazmine | University (OC) | 2:47.67 |
| 3 | SHOLAR, Caroline | University (OC) | 2:48.70 |
| 4 | COFFEE, Madeleine | Seminole (Sanford) | 2:51.28 |
| 5 | DAVID, Alaina | Hagerty | 2:54.11 |
| 6 | VERDU, Ashley | Lake Howell | 2:56.76 |
| 7 | TURNER, Athena | Hagerty | 3:04.11 |
| 8 | MAUGHAN, Pippa | Geneva School | 3:09.09 |
| 9 | BARRETT, Emery | Lake Brantley | 3:14.96 |
| 10 | WILT, Scarlett | Lyman | 3:15.00 |
| 11 | AMIRKHOSRAVI, Leila | Lake Brantley | 3:17.72 |
| 12 | RAGSDALE, Emma | Geneva School | 3:21.39 |
| 13 | FULLER, Layla | Lake Brantley | NT |