| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CURRY, Jerome | Oviedo | 55.00 |
| 2 | RICHARDS, Jayden | Lyman | 54.02 |
| 3 | WOODSON, Braxton | Lake Brantley | 51.55 |
| 4 | TURNER, Amari | Seminole (Sanford) | 48.39 |
| 5 | BLAKE, Jalen | Seminole (Sanford) | 50.50 |
| 6 | DAVIS, Jadlen | Lake Brantley | 53.89 |
| 7 | MCCOY, Alex | Oviedo | 54.83 |
| 8 | FORBES, Mason | Lake Howell | 55.14 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | SIGUENZA, Ryan | Oviedo | 56.74 |
| 2 | AYENDE-ORTIZ, Robert | University (OC) | 56.15 |
| 3 | AYALA, Anthony | Lake Brantley | 55.66 |
| 4 | ROBERTS, Jaiden | Winter Springs | 55.35 |
| 5 | HOLMES, Kelsie | Seminole (Sanford) | 55.58 |
| 6 | ERDMANN-DUNLAP, David | Hagerty | 56.06 |
| 7 | RHODES, James | Hagerty | 56.37 |
| 8 | JABER, Shadi | University (OC) | 57.76 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GOLSON, Torrell | Lake Howell | NT |
| 2 | SITDYKOV, DANIEL | Crooms | NT |
| 3 | GILAS, Anthony | Geneva School | 1:04.49 |
| 4 | LEPORE, Vincent | Lake Mary | 58.12 |
| 5 | LEGRAND, Devon | Lake Mary | 58.34 |
| 6 | MARTORANA, Elias | Winter Springs | 1:05.15 |
| 7 | SCHROEDER, Jack | Lake Howell | NT |
| 8 | CANNON, Elijah | Lyman | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | VINIKAS, NOAH | Crooms | NT |
| 3 | VOLLENWEIDER, Aidan | Geneva School | NT |
| 4 | SHYY, Dylan | Hagerty | NT |
| 5 | SIMMONS, Qamari | Lyman | NT |
| 6 | RILEY, A'sean | Lake Mary | NT |
| 7 | QUINTANA, Elijah | Geneva School | NT |