| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BATTERSON, Ben | Winter Park | NT | |
| 3 | RODRIGUEZ, Jaden | Trinity Christian (Deltona) | 52.16 | Deltona HS Senior Night Meet |
| 4 | DE NOBREGA, Regan | Faith Christian | 48.85 | Astro Last Chance Meet |
| 5 | THOMAS, Brian | Orlando Christian Prep | 50.31 | Highlander Classic 2022 |
| 6 | PINI, RJ | Trinity Prep | 58.52 | Carl Friedland Memorial |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SMALL, Sinsear | Pine Ridge | 47.86 | Deltona HS Senior Night Meet |
| 2 | POLCARO, Leonard | Montverde Academy | 47.55 | Bolles Bulldog Classic |
| 3 | NEW, Grayson | Pine Ridge | 46.27 | Volusia/Flagler Fr/So Meet |
| 4 | LEWIS, Justin | Victory Christian (LL) | 46.04 | Calvary Christian Invitational |
| 5 | DESSOURCES, Marc | Pine Ridge | 46.07 | George Janan SC Invitational M |
| 6 | SAYA, Tristan | Faith Christian | 47.12 | Mount Dora Christian Invitational Meet #1 |
| 7 | JOSEPH JR, Mackeny | Faith Christian | 47.56 | Mount Dora Christian Invitational Meet #1 |
| 8 | SWEENEY, Ladanian | Bishop Moore | 48.02 | Carl Friedland Memorial |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DEMOSTHENES, Joshua | Orlando Christian Prep | 44.84 | Dr. Phillips Panther Invite |
| 2 | PARROW, Adian | Victory Christian (LL) | 44.06 | Lakeland City Meet 2022 |
| 3 | GUNN, Zion | Winter Park | 41.73 | Metro East Conference Championships |
| 4 | TURNER, James | Winter Park | 38.07 | Dr. Phillips Panther Invite |
| 5 | JOHNSON JR., Cedric | Lake Highland Prep | 40.98 | Tiger Invitational |
| 6 | ADORNETTO, Antonio | Bishop Moore | 44.05 | Highlander Classic 2022 |
| 7 | MCGRATH, Joel | Victory Christian (LL) | 44.47 | Calvary Christian Invitational |
| 8 | RAWLINGS, Warren | Bishop Moore | 45.15 | Larry Kelly Invite |