| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | GUNN, Aalyssa | Orlando Christian Prep | NT | |
| 4 | SANZO, Isabella | Victory Christian (LL) | 1:02.32 | Calvary Christian Invitational |
| 5 | WEST, Jenna | Victory Christian (LL) | 1:03.18 | Highlander Classic 2022 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HARDEN, Trinity | Orlando Christian Prep | NT | |
| 2 | DIX, Sasha | Faith Christian | 59.81 | Astro Last Chance Meet |
| 3 | YARED, Lindsey | Trinity Prep | 55.78 | Carl Friedland Memorial |
| 4 | BARTIMUS, Emily | Winter Park | 54.50 | |
| 5 | EARLY, Hayden | Bishop Moore | 55.58 | Carl Friedland Memorial |
| 6 | CLEMONS, Caitlin | Winter Park | 56.23 | Metro East Conference Championships |
| 7 | STITES, Emma | Trinity Christian (Deltona) | 1:00.69 | Deltona HS Senior Night Meet |
| 8 | BRENAY, Natalie | Pine Ridge | 1:02.35 | Deltona HS Senior Night Meet |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GROOM, Sofia | Bishop Moore | 53.90 | Carl Friedland Memorial |
| 2 | MASSEY, Janiah | Pine Ridge | 52.90 | Deltona HS Senior Night Meet |
| 3 | MILIOTES, Anna | Trinity Prep | 51.55 | Highlander Classic 2022 |
| 4 | HEARD, Maya | Pine Ridge | 51.14 | Deltona HS Season Opener |
| 5 | LANE, Claudia | Winter Park | 51.54 | Dr. Phillips Panther Invite |
| 6 | CHOQUETTE, Amber | Victory Christian (LL) | 51.88 | Highlander Classic 2022 |
| 7 | ADCOCK, Raylynn | Trinity Christian (Deltona) | 53.66 | Deltona HS Senior Night Meet |
| 8 | YARED, Hannah | Trinity Prep | 54.45 | Lake Mary Invitational 2022 |