| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BRADY, Grace | Trinity Prep | NT | |
| 3 | BRANGERS, Kyrie | Victory Christian (LL) | 1:18.40 | Calvary Christian Invitational |
| 4 | LEWIS, Aaliyah | Orlando Christian Prep | 1:16.17 | Boone Invitational 2022 |
| 5 | SHEPHERD, Alayna | Geneva School | 1:17.72 | Mount Dora Christian Invitational Meet #1 |
| 6 | RODRIGUEZ, Alyssa | Faith Christian | 1:24.59 | East River Invitational |
| 7 | BONNEWITZ, Paloma | Bishop Moore | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BLANTON, Kate | Geneva School | 1:13.38 | East River Invitational |
| 2 | APRILL, Evia | Lake Mary Prep | 1:08.06 | Mount Dora Developmental Meet #2 |
| 3 | MALABUYO, Ella | Trinity Prep | 1:06.06 | Highlander Classic 2022 |
| 4 | WILCOX, Kara | Trinity Prep | 1:05.03 | Highlander Classic 2022 |
| 5 | YOERGER, Alyssa | Bishop Moore | 1:06.00 | |
| 6 | TATTRIE, Abby | Victory Christian (LL) | 1:07.92 | Highlander Classic 2022 |
| 7 | DIX, Sasha | Faith Christian | 1:12.26 | Mount Dora Christian Invitational Meet #1 |
| 8 | SAVIOUS, Mia | Pine Ridge | 1:14.72 | Deltona HS Senior Night Meet |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BERNARD, Patley | Winter Park | 1:04.79 | Boone Invitational 2022 |
| 2 | DAY, Jani | Winter Park | 1:01.81 | Boone Invitational 2022 |
| 3 | OQUENDO, Eliana | Winter Park | 1:01.65 | Boone Invitational 2022 |
| 4 | WILLIAMS, Alivia | Montverde Academy | 56.44 | Florida State Relays |
| 5 | ORPHELIA, Kiarra | Montverde Academy | 1:00.40 | Mount Dora Christian Invitational Meet #1 |
| 6 | BROWN, Siarah | Orlando Christian Prep | 1:01.74 | Boone Invitational 2022 |
| 7 | LEMMOND, Addie | Victory Christian (LL) | 1:04.66 | Shorecrest Quad Meet |
| 8 | GELLNER, Madison | Bishop Moore | 1:05.00 |