| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Montverde Academy | A | Brauman | Barbaza | Recinos | Nesmith | 9:47.89 | 2018 FHSAA State Track & Field Championships |
| 2 | Lake Highland Prep | A | Bell | Richards | Newman | Johnson | 10:08.00 | Highlander Classic |
| 3 | Master's Academy (Oviedo) | A | Wyant | Register | Merly | Newman | 11:18.78 |