| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Brady, Grace | FR | Trinity Prep | 1:16.15 | 46th Montverde Eagle Invitational 2021 |
| 2 | Nesmith, Natalie | SO | Montverde Academy | 1:10.42 | 46th Montverde Eagle Invitational 2021 |
| 3 | Miranda, Mirabella | SR | Windermere Prep | 1:07.42 | Highlander Classic @ LHP 2021 |
| 4 | Kahn, Carly | 7 | Lake Highland Prep | 1:07.00 | |
| 5 | Desai, Kayla | SO | Lake Highland Prep | 1:07.22 | Windermere Meet of Champions 2021 |
| 6 | Hudson, Hannah | SO | First Academy (Orlando) | 1:07.57 | Highlander Classic @ LHP 2021 |
| 7 | Colombo, Avery | JR | Lake Highland Prep | 1:15.13 | Royal Invitational @ TFA 2021 |
| 8 | Henderson, Eleanore | FR | Trinity Prep | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Wilson, Ryleigh | FR | Master's Academy (Oviedo) | 1:05.33 | Saints Invitational 2019 |
| 2 | Saranita , Chloe | 7 | Montverde Academy | 1:03.50 | TCCL - MS Championship |
| 3 | McMullen , Madison | SR | Montverde Academy | 1:02.00 | |
| 4 | Jennings, Jaz'Myne | FR | Montverde Academy | 57.00 | |
| 5 | Brown, Siarah | SO | Orlando Christian Prep | 1:00.59 | East River Invitational 2021 |
| 6 | Wright, Jasmyne | JR | Orlando Christian Prep | 1:02.60 | 2019 FHSAA State Track & Field Championships |
| 7 | Wilson, Alexa | JR | Master's Academy (Oviedo) | 1:03.65 | |
| 8 | Gunn, Aalyssa | SO | Orlando Christian Prep | 1:06.38 | Winter Haven Invitational |