| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DRAWDY, Mya | Calvary Christian (PtStL) | 3:03.11 | Jensen Beach Invitational 2025 |
| 2 | BREWER, Blaire | Duval Charter Baymeadows |
3:14.38 | Tocoi Creek Toro Showdown 2025 (Approved Qualifier) |
| 3 | MORALES, Novalee | Calvary Christian (PtStL) | 3:16.23 | Bulldog Invitational 2025 |
| 4 | MORALES, Annalise | Calvary Christian (PtStL) | 3:16.60 | Bulldog Invitational 2025 |
| 5 | MARTIN, Juliet | Master's Academy (VB) |
3:21.94 | Kowboy Invitational |
| 6 | DORADO, Rocio | Duval Charter Baymeadows |
3:36.23 | Panther Invite at Ridgeview High School |
| 7 | MARTINS, Sophia | Master's Academy (VB) |
4:06.53 | Pineapple Cove MS Track 1 2025 |
| 8 | LIRIANO, Lindsay | Foundation Christian |
NT | NT |
| 9 | WEBB, Makena | Indian Rocks Christian |
NT | NT |
| 10 | RAMIREZ, Kaela | Cambridge Christian |
NT | NT |
| 11 | MARSHALL, Audrey | Foundation Christian |
NT | 2:55 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LUBKE, Adelyn | Indian Rocks Christian |
2:26.73 | Calvary Christian Warrior Invitational |
| 2 | BUDWICK, Kaiya | Cambridge Christian |
2:27.06 | RADD Sunshine Showcase High School |
| 3 | MEISTER, Genevieve | Cambridge Christian |
2:32.70 | Florida High School Indoor Championships |
| 4 | BONHAM, Sage | Indian Rocks Christian |
2:40.62 | Shorecrest Senior Night |
| 5 | TRESSELL, Dafni | Babcock | 2:49.35 | Saint Stephens Falcon Invite 2025 |
| 6 | BEERS, Lizzie | Babcock | 2:50.30 | 65th Annual Edison Relays |
| 7 | WOODS, Shiloh | Classical Academy (Sarasota) |
2:56.94 | North Port Invitational 2025 |
| 8 | SCHUITEMAN, Bailey | Bradenton Christian |
2:57.13 | Hurricane Invitational Track and Field Meet |
| 9 | COLLAZO, Jaylene | Babcock | 2:58.03 | 65th Annual Edison Relays |