| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BONFIG, Sophie | Babcock | NT | NT |
| 3 | FERRY, Meikah | Babcock | 1:24.91 | Saint Stephens Falcon Invite 2025 |
| 4 | HELM, Ella | Indian Rocks Christian |
1:04.87 | Calvary Christian Warrior Invitational |
| 5 | LUBKE, Elliana | Indian Rocks Christian |
1:14.46 | Calvary Christian Warrior Invitational |
| 6 | DEVERMANN, Aly | Babcock | 1:27.69 | Lakewood Ranch Invitational 2025 |
| 7 | HODGES, Rebecca | Duval Charter Baymeadows |
NT | NT |