| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | First Academy (Orlando) |
B | 47.13 |
| 2 | First Academy (Orlando) |
A | 45.56 |
| 3 | Lake Minneola |
A | 44.81 |
| 4 | Rockledge |
A | 41.99 |
| 5 | Eustis |
A | 43.81 |
| 6 | Lake Nona |
A | 45.34 |
| 7 | Lake Buena Vista |
A | 46.38 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | First Academy (Orlando) |
A | NT | |
| 2 | Lake Buena Vista |
B | DNS | Timber Creek Quad Meet |
| 3 | Windermere |
A | DNF | Windermere Meet of Champions Classic 2024 |
| 4 | Rockledge |
A | 42.28 | Satellite High Reggie Johnson Memorial |
| 5 | Eustis |
A | 43.54 | Willdwood Wildcat Fast on the First |
| 6 | Cocoa |
A | DNF | Cocoa Tigers Time Trials |
| 7 | Florida Prep |
A | DNS | Holy Trinity Invitational powered by Track Legend |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | First Academy (Orlando) |
B | NT | |
| 2 | Lake Buena Vista |
A | 46.10 | Timber Creek Quad Meet |
| 3 | Lake Minneola |
A | 43.65 | 2024 Be Like Ike Invitational (meet is full) |
| 4 | Edgewater |
A | DNS | Bishop Moore - Larry Kelly Invite 2024 (Regional Qualifier) |
| 5 | Bartow |
A | DNS | Davenport - Bronco Invitational 2024 |
| 6 | Holy Trinity |
A | DNS | Holy Trinity Invitational powered by Track Legend |
| 7 | Geneva School |
A | DNS | Mount Dora Christian Invitational |
| 8 | Lake Nona |
A | NT |