| Lane | Team | Squad | Seed | 
|---|---|---|---|
| 1 |  First Academy (Orlando) | 
		B | 47.13 | 
| 2 |  First Academy (Orlando) | 
		A | 45.56 | 
| 3 |  Lake Minneola | 
		A | 44.81 | 
| 4 |  Rockledge | 
		A | 41.99 | 
| 5 |  Eustis | 
		A | 43.81 | 
| 6 |  Lake Nona | 
		A | 45.34 | 
| 7 |  Lake Buena Vista | 
		A | 46.38 | 
| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 1 |  First Academy (Orlando) | 
		A | NT | |
| 2 |  Lake Buena Vista | 
		B | DNS | Timber Creek Quad Meet | 
| 3 |  Windermere | 
		A | DNF | Windermere Meet of Champions Classic 2024 | 
| 4 |  Rockledge | 
		A | 42.28 | Satellite High Reggie Johnson Memorial | 
| 5 |  Eustis | 
		A | 43.54 | Willdwood Wildcat Fast on the First | 
| 6 |  Cocoa | 
		A | DNF | Cocoa Tigers Time Trials | 
| 7 |  Florida Prep | 
		A | DNS | Holy Trinity Invitational powered by Track Legend | 
| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 1 |  First Academy (Orlando) | 
		B | NT | |
| 2 |  Lake Buena Vista | 
		A | 46.10 | Timber Creek Quad Meet | 
| 3 |  Lake Minneola | 
		A | 43.65 | 2024 Be Like Ike Invitational (meet is full) | 
| 4 |  Edgewater | 
		A | DNS | Bishop Moore - Larry Kelly Invite 2024 (Regional Qualifier) | 
| 5 |  Bartow | 
		A | DNS | Davenport - Bronco Invitational 2024 | 
| 6 |  Holy Trinity | 
		A | DNS | Holy Trinity Invitational powered by Track Legend | 
| 7 |  Geneva School | 
		A | DNS | Mount Dora Christian Invitational | 
| 8 |  Lake Nona | 
		A | NT |