| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 1 | CARTER, Kenneth |  Rockledge | 
		59.43 | 
| 2 | WARREN, Joshua |  Lake Nona | 
		DNS | 
| 3 | FREEMAN, Amari |  Rockledge | 
		57.22 | 
| 4 | BASS, Logan |  Cocoa | 
		56.86 | 
| 5 | MCROY, Camryn |  Holy Trinity | 
		DNS | 
| 6 | SMITH, Kenneth |  Lake Minneola | 
		57.31 | 
| 7 | SIMMONS-ROBERTS, Tavon |  Cocoa | 
		58.20 | 
| 8 | CHO-SAM, Zackary |  Mount Dora | 
		59.73 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | DE NOBREGA, Regan |  Faith Christian | 
		NT | NT | 
| 2 | SAYA, Tristan |  Faith Christian | 
		1:07.16 | East River Falcon Takeoff | 
| 3 | JOHNSON, Jonathan |  Eustis | 
		1:02.35 | Willdwood Wildcat Fast on the First | 
| 4 | BASS, Logan |  Cocoa | 
		57.02 | Davenport - Bronco Invitational 2024 | 
| 5 | BALLARD, Jaylen |  Holy Trinity | 
		1:00.66 | Holy Trinity Invitational powered by Track Legend | 
| 6 | HUGHES, Walker |  First Academy (Orlando) | 
		1:06.19 | Winter Park - Wildcat Open 2024 | 
| 7 | RHODES, Tyler |  Bartow | 
		1:15.14 | Davenport - Bronco Invitational 2024 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | CHO-SAM, Zackary |  Mount Dora | 
		NT | 59.01 | 
| 2 | HAY, Demetre |  Windermere | 
		1:07.41 | Windermere Meet of Champions Classic 2024 | 
| 3 | ISOM, Ethan |  Lake Minneola | 
		1:02.61 | Willdwood Wildcat Invitational | 
| 4 | FREEMAN, Amari |  Rockledge | 
		57.87 | Satellite High Reggie Johnson Memorial | 
| 5 | MCROY, Camryn |  Holy Trinity | 
		1:00.43 | Holy Trinity Invitational powered by Track Legend | 
| 6 | GLOVER, Deontae |  Cocoa | 
		1:05.98 | Davenport - Bronco Invitational 2024 | 
| 7 | TIKHONOV, Fedir |  Lake Buena Vista | 
		1:12.53 | St. Cloud Invitational | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | IVSIC, Miles |  First Academy (Orlando) | 
		1:09.09 | Winter Park - Wildcat Open 2024 | 
| 3 | SIMMONS-ROBERTS, Tavon |  Cocoa | 
		1:03.27 | Davenport - Bronco Invitational 2024 | 
| 4 | WARREN, Joshua |  Lake Nona | 
		58.11 | George Jenkins - Bob English Invitational 2024 | 
| 5 | OQUENDO-WATERS, Amari |  Rockledge | 
		59.33 | St. Cloud Invitational | 
| 6 | COLEMAN, Anthony |  Rockledge | 
		1:05.18 | Satellite High Reggie Johnson Memorial | 
| 7 | FOGA, Fabiano |  Windermere | 
		1:10.36 | Windermere Meet of Champions Classic 2024 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | WILLIAMS, Tony |  Eustis | 
		1:09.90 | Willdwood Wildcat Fast on the First | 
| 3 | MCDONALD, Marquis |  Lake Minneola | 
		1:03.51 | 2024 River City Relays Classic (Sanctioned Meet) | 
| 4 | CARTER, Kenneth |  Rockledge | 
		58.40 | Satellite High Reggie Johnson Memorial | 
| 5 | SMITH, Kenneth |  Lake Minneola | 
		58.44 | 2024 River City Relays Classic (Sanctioned Meet) | 
| 6 | NEVILLE, Anthony |  Lake Buena Vista | 
		1:03.66 | St. Cloud Invitational | 
| 7 | WALKER, Amir |  Eustis | 
		1:10.05 | Willdwood Wildcat Fast on the First |