| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Port | A | Duque | Gallant | Sabo | Crane | 8:17.11 | FHSAA Class 4A & Para Track & Field State Championships |
| 2 | Out-of-Door | A | Iturralde | Roberts | Beck | Douglas | 8:32.26 | IMG Academy Invitational |
| 3 | Lakewood Ranch | A | Johnson | Leatt | Robinson | Rivera | 8:45.00 | |
| 4 | Riverview (Sarasota) | A | Fenderson | Holmes | Thomas | Quintero | 8:50.00 | |
| 5 | Cardinal Mooney | A | Yencik | Taylor | Morton | Hassler | 8:55.76 | FHSAA 1A District 11 |
| 6 | Manatee | A | Schultz | Rickert | Mckeaver | Lages | 10:00.00 | |
| 7 | Venice | A | Calvillo Camacho | Evans | Nuttall | Young | 10:00.00 | |
| 8 | Tampa Bay HEAT | A | Mullen | Poe | Beebe | Rill | NT |