| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | CAMPBELL, Harper | Out-of-Door | NT | |
| 3 | BARTON, Mikaela | Braden River | NT | |
| 4 | MARTIN, Aline | Venice | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JACKSON, Daisia | Wharton | NT | |
| 2 | SANON, Wes | Hardee | NT | |
| 3 | HAMMOND, Elizabeth | Wharton | 1:06.27 | 5th Annual Cypress Creek Invitational |
| 4 | HYLAND, Danariah | Hardee | 1:07.29 | Lake Placid Meet #2 |
| 5 | ANDREWS, Brooke | Lemon Bay | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CARLIN, Haley | Riverview (Sarasota) | 1:03.95 | North Port Invite 2022 |
| 2 | ALEXANDER, Vivian | Out-of-Door | 1:01.49 | Spring Break Invitational at IMG |
| 3 | STERLING, Khiya | Charlotte | 1:00.23 | Charlotte Invitational |
| 4 | SOUNDARA, Nirvana | Palmetto | 1:00.64 | Larry Richardson Memorial Invite |
| 5 | CEASER, Caroline | Riverview (Sarasota) | 1:01.72 | Spring Break Invitational at IMG |
| 6 | DE LOS SANTOS, Shantal | Lemon Bay | 1:04.10 | North Port Invite 2022 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | FOLKERS, Brianna | Lakewood Ranch | 59.37 | Palmetto Invite |
| 2 | SANTIAGO, Sofia | Booker | 58.19 | Larry Richardson Memorial Invite |
| 3 | MCNINCH, Gracyn | Lakewood Ranch | 54.73 | |
| 4 | ALFLEN, Kassidy | Braden River | 57.45 | Palmetto Invite |
| 5 | SEBIRO, Chloe | Booker | 58.70 | Larry Richardson Memorial Invite |
| 6 | BUNDRAGE, Ronasia | Braden River | 59.62 | Braden River Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WATTS, Kalea | Palmetto | 54.65 | Larry Richardson Memorial Invite |
| 2 | CHERNIAK, Sophia | Lemon Bay | 53.13 | Larry Richardson Memorial Invite |
| 3 | JOHNSON, Niecey | Bradenton Christian | 50.00 | SSES Quad Meet |
| 4 | MURACK, Makena | Lakewood Ranch | 51.01 | Spring Break Invitational at IMG |
| 5 | IVANENKO, Milana | Venice | 53.83 | North Port Invite 2022 |
| 6 | IRELAND, Emily | Venice | 54.65 | North Port Invite 2022 |