| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Booker | A | NT | |
| 3 | Cardinal Mooney | A | 46.80 | Larry Richardson Memorial Invite |
| 4 | Out-of-Door | A | 46.93 | Bobcat Relays |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Bradenton Christian | A | 45.38 | Larry Richardson Memorial Invite |
| 3 | Lakewood Ranch | A | 45.03 | Braden River Invitational |
| 4 | Braden River | A | 45.14 | Braden River Invitational |
| 5 | Lemon Bay | A | 45.52 | North Port Invite 2022 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Charlotte | A | 44.80 | |
| 2 | Venice | A | 43.60 | |
| 3 | Palmetto | A | 42.80 | |
| 4 | Riverview (Sarasota) | A | 43.25 | North Port Invite 2022 |
| 5 | Wharton | A | DNS | 5th Annual Cypress Creek Invitational |
| 6 | Hardee | A | 44.81 | Lake Placid Meet #2 |